Artichoke: An Artist to Choke Cervical Cancer by Downregulation of Cyclin D and Bcl-2
Location
E4110
Document Type
Poster
Start Date
30-11-2023 12:15 PM
End Date
30-11-2023 12:55 PM
Description
Abstract
Background: Cervical cancer (CC) continues to be one of the leading causes of mortality worldwide. It is estimated that 90% of deaths from CC occur in low- and middle-income countries. Artichoke is a commonly consumed plant that has been studied more recently with increasing interest for its abundance in antioxidants. Our previous study showed that artichoke extract (AE) is a potent inhibitor of melanoma. To extend that study, this study is designed to assess the potential antitumor effects of AE on the SiHa CC cell line.
Methods: Clonogenic survival assay, cell proliferation, and caspase-3 activity kits were used to evaluate the effects of AE on cell survival, proliferation, and apoptosis of SiHa CC cells. Molecular mechanisms were further assessed by using RT-PCR and IHC.
Results: SiHa CC cell colony count significantly decreased in the presence of AE. A decrease in the OD value of CC cells was also found in the presence of AE. The relative caspase-3 activity in SiHa CC cells increased significantly in the presence of AE. The anti-proliferative effect of AE on SiHa CC cells correlated with decreased expression of cyclin D. The pro-apoptotic effect of AE on SiHa CC cells correlated with decreased expression of Bcl-2.
Conclusions: Artichoke inhibits growth of CC through inhibiting proliferation and promoting apoptosis by downregulation of cyclin D and Bcl-2. These findings extend the anti-tumor effect of artichoke from melanoma to CC, supporting the concept that artichoke exerts powerful anti-tumor property in not only one cancer. Such a study may be useful to develop natural treatments for many types of cancers.
Recommended Citation
Chadwick, William F.; Guan, Tianyun; Fang, Paris M.; Nelson, Christian; Mayberry, Trent; Cowan, Braydon; Wakefield, Mark R.; Dong, Lijun; and Fang, Yujiang, "Artichoke: An Artist to Choke Cervical Cancer by Downregulation of Cyclin D and Bcl-2" (2023). DMU Research Symposium. 8.
https://digitalcommons.dmu.edu/researchsymposium/2023rs/2023abstracts/8
Artichoke: An Artist to Choke Cervical Cancer by Downregulation of Cyclin D and Bcl-2
E4110
Abstract
Background: Cervical cancer (CC) continues to be one of the leading causes of mortality worldwide. It is estimated that 90% of deaths from CC occur in low- and middle-income countries. Artichoke is a commonly consumed plant that has been studied more recently with increasing interest for its abundance in antioxidants. Our previous study showed that artichoke extract (AE) is a potent inhibitor of melanoma. To extend that study, this study is designed to assess the potential antitumor effects of AE on the SiHa CC cell line.
Methods: Clonogenic survival assay, cell proliferation, and caspase-3 activity kits were used to evaluate the effects of AE on cell survival, proliferation, and apoptosis of SiHa CC cells. Molecular mechanisms were further assessed by using RT-PCR and IHC.
Results: SiHa CC cell colony count significantly decreased in the presence of AE. A decrease in the OD value of CC cells was also found in the presence of AE. The relative caspase-3 activity in SiHa CC cells increased significantly in the presence of AE. The anti-proliferative effect of AE on SiHa CC cells correlated with decreased expression of cyclin D. The pro-apoptotic effect of AE on SiHa CC cells correlated with decreased expression of Bcl-2.
Conclusions: Artichoke inhibits growth of CC through inhibiting proliferation and promoting apoptosis by downregulation of cyclin D and Bcl-2. These findings extend the anti-tumor effect of artichoke from melanoma to CC, supporting the concept that artichoke exerts powerful anti-tumor property in not only one cancer. Such a study may be useful to develop natural treatments for many types of cancers.
