Start Date
11-21-2024 12:15 PM
End Date
11-21-2024 12:55 PM
Description
Background: Bladder cancer is the second most common urological cancer in the world. The current treatment strategies involve surgery and chemotherapy; however, its 5-year survival rate is below 40% in more advanced cases. Cancer immunotherapy has become a popular approach to treating cancer and PDL1, PDL2, ICAM2 and 4-1BBL are key costimulatory molecules. Previous research has shown plant phytochemicals can act as immunomodulators by regulating co-stimulatory functions to increase T cell activation, inhibiting malignant proliferation. However, the role of aloe vera in the growth of bladder cancer and expression of key costimulatory molecules has not been studied yet.
Methods: Clonogenic survival assay was used to evaluate growth of a widely used bladder cancer cell line, T24, in the presence or absence of aloe vera extract. RT-PCR and IHC were performed to investigate the expression of PDL1, PDL2, ICAM2 and 4-1BBL.
Results: The percentage of colonies of T24 bladder cancer cells significantly decreased after treatment with aloe vera. Expression levels of PDL1 and PDL2 pro-oncogenic primers were downregulated, and anti-oncogenic primers ICAM-2 and 4-1BBL were upregulated in the presence of aloe vera.
Conclusions: Aloe inhibits bladder cancer cell growth and modulates expression of PDL1, PDL2, ICAM-2 and 4-1BBL. This suggests an immunomodulatory effect of aloe vera that can be exploited to develop new immunotherapies and warrants further investigation in bladder cancer and beyond.
Citation Information
Gutiu, Arturo; Marrah, Austin; Mayberry, Trent; Cowan, Braydon; Voss, Brady; Wakefield, Mark R.; and Fang, Yjiang, "Aloe vera constrains growth of bladder cancer and modulates expression of key costimulatory molecules" (2024). Office of Research DMU Research Symposium. 1.
https://digitalcommons.dmu.edu/researchsymposium/2024rs/2024abstracts/1
Aloe vera constrains growth of bladder cancer and modulates expression of key costimulatory molecules
Background: Bladder cancer is the second most common urological cancer in the world. The current treatment strategies involve surgery and chemotherapy; however, its 5-year survival rate is below 40% in more advanced cases. Cancer immunotherapy has become a popular approach to treating cancer and PDL1, PDL2, ICAM2 and 4-1BBL are key costimulatory molecules. Previous research has shown plant phytochemicals can act as immunomodulators by regulating co-stimulatory functions to increase T cell activation, inhibiting malignant proliferation. However, the role of aloe vera in the growth of bladder cancer and expression of key costimulatory molecules has not been studied yet.
Methods: Clonogenic survival assay was used to evaluate growth of a widely used bladder cancer cell line, T24, in the presence or absence of aloe vera extract. RT-PCR and IHC were performed to investigate the expression of PDL1, PDL2, ICAM2 and 4-1BBL.
Results: The percentage of colonies of T24 bladder cancer cells significantly decreased after treatment with aloe vera. Expression levels of PDL1 and PDL2 pro-oncogenic primers were downregulated, and anti-oncogenic primers ICAM-2 and 4-1BBL were upregulated in the presence of aloe vera.
Conclusions: Aloe inhibits bladder cancer cell growth and modulates expression of PDL1, PDL2, ICAM-2 and 4-1BBL. This suggests an immunomodulatory effect of aloe vera that can be exploited to develop new immunotherapies and warrants further investigation in bladder cancer and beyond.
