Grid1 expression in female rats at 42 days withdrawal from chronic intermittent ethanol exposure
Description
Substance use disorders are chronic health disorders that contribute a large burden on individuals and the health care system. Understanding how these use disorders develop, change, and are maintained across the life span of individuals will aid in the development of treatment plans and possible therapeutics. It is known that during withdrawal from drug use, functional alterations occur that predispose an individual’s risk to return to drug use. The current study seeks to identify if any alterations in the structure of neuronal communication sites plays a role in the development or expression of functional increases that affect relapse susceptibility. As such, we are exploring the expression of Grid1, a delta type glutamate receptor, that plays a role in the formation of synaptic contacts between neurons. We hypothesize that Grid1 is dynamically regulated in response to withdrawal state dependent alterations in glutamate receptor function. To conduct these studies, female rats were exposed to ethanol vapor and then placed into forced abstinence of 42 days. Brains were then extracted and treated with sulfo-NHS-S-S-Biotin to label extracellular proteins. Using western blot techniques and Grid1 antibodies, we are measuring the relative amount of protein expression of pyramidal neurons in our samples in comparison to control animals. Preliminary data is still being calculated.
Citation Information
Ogihara, Toscana; Giles, Jennifer; Boyer, Michael; and Christian, Daniel T., "Grid1 expression in female rats at 42 days withdrawal from chronic intermittent ethanol exposure" (2026). Office of Research DMU Research Symposium. 66.
https://digitalcommons.dmu.edu/researchsymposium/2025rs/2025abstracts/66
Grid1 expression in female rats at 42 days withdrawal from chronic intermittent ethanol exposure
Substance use disorders are chronic health disorders that contribute a large burden on individuals and the health care system. Understanding how these use disorders develop, change, and are maintained across the life span of individuals will aid in the development of treatment plans and possible therapeutics. It is known that during withdrawal from drug use, functional alterations occur that predispose an individual’s risk to return to drug use. The current study seeks to identify if any alterations in the structure of neuronal communication sites plays a role in the development or expression of functional increases that affect relapse susceptibility. As such, we are exploring the expression of Grid1, a delta type glutamate receptor, that plays a role in the formation of synaptic contacts between neurons. We hypothesize that Grid1 is dynamically regulated in response to withdrawal state dependent alterations in glutamate receptor function. To conduct these studies, female rats were exposed to ethanol vapor and then placed into forced abstinence of 42 days. Brains were then extracted and treated with sulfo-NHS-S-S-Biotin to label extracellular proteins. Using western blot techniques and Grid1 antibodies, we are measuring the relative amount of protein expression of pyramidal neurons in our samples in comparison to control animals. Preliminary data is still being calculated.
