Therapeutic potential of G1 on renal blood flow and tissue oxygenation in an experimental model of sleep apnea.
Description
Introduction: Sleep apnea (SA), a condition characterized by recurrent cessation of breathing during sleep is recognized as an independent risk factor for development of hypertension (HTN). Epidemiological studies show HTN risk is lower in pre-menopausal women with SA relative to similarly aged men with SA, however the risk for women increases after menopause. Previous studies have demonstrated that vascular dysfunction and heightened renal sympathetic nerve activity contributes to the pathophysiology of hypertension and renal dysfunction in experimental models of SA. Our lab has previously shown that estrogen loss in female rats via ovariectomy (OVX) results in reductions in renal blood flow (RBF) and tissue oxygenation (PO2) in rats exposed to chronic intermittent hypoxia (CIH, experimental model of SA). In the current study, we hypothesized that activation of a specific estrogen receptor (GPER, g-protein coupled estrogen receptor) with a selective agonist (G1) would attenuate previously observed decreases in RBF and PO2 in OVX females conditioned with CIH. Methods: To address this hypothesis 2 groups of adult female Sprague Dawley rats were conditioned with CIH or a control condition (21% oxygen) for 14 days while receiving treatment with G1 via osmotic mini pumps. At the conclusion of the conditioning period, RBF and PO2 were measured under light isoflurane anesthesia (1.5-2% in air). Discussion: Analysis is ongoing, but we expect that RBF and PO2 will be similar between groups exposed to AIR and CIH due to a salutary effect of treatment with G1.
Citation Information
Lombardi Labegaline, Julia; Ordaz, Daniel; Watson, Jackson; Lin, Emma; Kleine, Ryan; Kayser, Anna; Clayton, Sarah; and Marcus, Noah, "Therapeutic potential of G1 on renal blood flow and tissue oxygenation in an experimental model of sleep apnea." (2026). Office of Research DMU Research Symposium. 68.
https://digitalcommons.dmu.edu/researchsymposium/2025rs/2025abstracts/68
Therapeutic potential of G1 on renal blood flow and tissue oxygenation in an experimental model of sleep apnea.
Introduction: Sleep apnea (SA), a condition characterized by recurrent cessation of breathing during sleep is recognized as an independent risk factor for development of hypertension (HTN). Epidemiological studies show HTN risk is lower in pre-menopausal women with SA relative to similarly aged men with SA, however the risk for women increases after menopause. Previous studies have demonstrated that vascular dysfunction and heightened renal sympathetic nerve activity contributes to the pathophysiology of hypertension and renal dysfunction in experimental models of SA. Our lab has previously shown that estrogen loss in female rats via ovariectomy (OVX) results in reductions in renal blood flow (RBF) and tissue oxygenation (PO2) in rats exposed to chronic intermittent hypoxia (CIH, experimental model of SA). In the current study, we hypothesized that activation of a specific estrogen receptor (GPER, g-protein coupled estrogen receptor) with a selective agonist (G1) would attenuate previously observed decreases in RBF and PO2 in OVX females conditioned with CIH. Methods: To address this hypothesis 2 groups of adult female Sprague Dawley rats were conditioned with CIH or a control condition (21% oxygen) for 14 days while receiving treatment with G1 via osmotic mini pumps. At the conclusion of the conditioning period, RBF and PO2 were measured under light isoflurane anesthesia (1.5-2% in air). Discussion: Analysis is ongoing, but we expect that RBF and PO2 will be similar between groups exposed to AIR and CIH due to a salutary effect of treatment with G1.
